Modeling of In Vivo Tissue Electroporation and Cellular Uptake Enhancement

Finite Element Modeling of in Vivo Electroporation

Numerical modeling for in vivo electroporation.

Electropermeabilization of cell plasma membrane is a threshold phenomenon. When a cell is exposed to electric field a spatially dependent transmembrane potential is induced (1). Above a certain threshold value of transmembrane potential permeability of plasma membrane drastically increases. Thus, in order to obtain plasma membrane permeabilization an above threshold transmembrane potential need...

Cellular senescence and tissue aging in vivo.

A long-standing controversy concerns the relevance of cellular senescence, defined and observed as a cell culture phenomenon, to tissue aging in vivo. Here the evidence on this topic is reviewed. The main conclusions are as follows. First, telomere shortening, the principal known mediator of cellular senescence, occurs in many human tissues in aging. Second, it is not clear whether this results...

In Vivo Cellular Uptake, Degradation, and Biocompatibility of Polyelectrolyte Microcapsules**

There has been a growing interest in the design and development of polymeric microspheres as controlled release systems for drugs and antigens. Different types of polymers have been used to design biodegradable polymeric microparticles. Poly(lactic-co-glycolic) acid (PLGA) microspheres have been extensively investigated because of their biocompatibility and biodegradability. PLGA has been appro...

Optimized In Vivo Transfer of Small Interfering RNA Targeting Dermal Tissue Using In Vivo Surface Electroporation

Electroporation (EP) of mammalian tissue is a technique that has been used successfully in the clinic for the delivery of genetic-based vaccines in the form of DNA plasmids. There is great interest in platforms which efficiently deliver RNA molecules such as messenger RNA and small interfering RNA (siRNA) to mammalian tissue. However, the in vivo delivery of RNA enhanced by EP has not been exte...